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  • Cell Culture Technology
    Cell Culture Technology

    This textbook provides an overview on current cell culture techniques, conditions, and applications specifically focusing on human cell culture.This book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts, and therefore delivers in a compact and clear way important theoretical, as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. The book is written for Master students and PhD candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. It will be of interest to doctoral colleges, Master- and PhD programs teaching courses in this area of research.

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  • Mouse Cell Culture : Methods and Protocols
    Mouse Cell Culture : Methods and Protocols

    This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems.After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages.Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets.

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  • Cell Culture Engineering : Recombinant Protein Production
    Cell Culture Engineering : Recombinant Protein Production

    Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology.They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology.It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns.Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production.In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.

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  • Which travel destination causes wanderlust when traveling alone?

    Traveling alone to destinations like Bali, Indonesia, or the Amalfi Coast in Italy can cause wanderlust. These places offer stunning natural beauty, rich cultural experiences, and opportunities for self-discovery. The freedom to explore at your own pace and immerse yourself in the local culture can create a sense of wanderlust and adventure. Additionally, the chance to meet new people and make meaningful connections can add to the allure of these destinations for solo travelers.

  • Which travel destination do you recommend for an adventure vacation in 2022?

    I recommend Iceland as a travel destination for an adventure vacation in 2022. With its stunning landscapes including volcanoes, glaciers, hot springs, and waterfalls, Iceland offers a wide range of outdoor activities such as hiking, glacier trekking, snorkeling in crystal-clear waters, and exploring ice caves. Additionally, you can witness the mesmerizing Northern Lights during the winter months. Iceland's unique natural beauty and thrilling adventures make it a perfect choice for an unforgettable adventure vacation in 2022.

  • How does the process of cell culture technology proceed?

    Cell culture technology involves the growth and maintenance of cells outside of their natural environment, typically in a laboratory setting. The process begins with the selection of the appropriate cell type and the preparation of a suitable growth medium. The cells are then isolated from their natural environment and placed in a sterile culture vessel, where they are provided with the necessary nutrients and conditions for growth. The culture is regularly monitored and maintained to ensure the cells remain healthy and continue to proliferate. This technology is widely used in research, drug development, and biotechnology for studying cell behavior, producing biological products, and testing potential therapies.

  • Rhodes sightseeing travel guide

    Rhodes is a beautiful island in Greece with a rich history and stunning architecture. Some must-see sights include the Palace of the Grand Master of the Knights of Rhodes, the ancient Acropolis of Lindos, and the charming Old Town with its medieval walls and cobblestone streets. Don't miss the Valley of the Butterflies, a unique natural reserve where thousands of butterflies gather in the summer months. Make sure to also visit the picturesque village of Lindos and its iconic clifftop acropolis for breathtaking views of the Aegean Sea.

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  • Organ Regeneration : 3D Stem Cell Culture & Manipulation
    Organ Regeneration : 3D Stem Cell Culture & Manipulation

    This volume provides readers with a better understanding of organogenesis in developmental biology and next-generation organ regenerative therapy.This book focuses on recent studies of organ regeneration from stem cells using in vitro 3D cell culture and manipulation.The chapters cover topics such as generation of a 3D retinal tissue formation; functional salivary gland regeneration; liver regeneration using cultures liver bud; and in vivo model of small intestine.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Organ Regeneration: 3D Stem Cell Culture and Manipulation is a valuable resource for scientists and researchers who are interested in this field.

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  • Exploration and Discovery
    Exploration and Discovery

    The discovery and mapping of the world, its peoples, oceans and continents, are the result of centuries of exploration.This book traces the history of such travel and the pioneers who pushed back the frontiers of the known world.It explains the reasons for their journeys, whether out of scientific curiosity, to create trading opportunities, to spread religious beliefs or for personal gain or glory.With the aid of over 250 illustrations, maps and photographs, this accessible reference work captures all the excitement and spirit of adventure.

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  • Wanderlust: Travel Stories
    Wanderlust: Travel Stories

    In a bar, on the world's most remote island, a group of strangers share their stories. Step into their shoes and experience travel from distinct perspectives...

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  • Kids Adventure Toys Kit 14PCS Outdoor Nature Exploration Toys Elescope Dragonfly Butterfly Catching
    Kids Adventure Toys Kit 14PCS Outdoor Nature Exploration Toys Elescope Dragonfly Butterfly Catching

    Kids Adventure Toys Kit 14PCS Outdoor Nature Exploration Toys Elescope Dragonfly Butterfly Catching

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  • Rhodes Sightseeing Travel Guide

    The Rhodes Sightseeing Travel Guide is a comprehensive resource for visitors looking to explore the beautiful island of Rhodes in Greece. It provides detailed information on popular attractions, historical sites, and cultural landmarks, as well as practical tips for getting around the island. The guide also includes recommendations for dining, shopping, and accommodations to help travelers make the most of their visit to Rhodes. Whether you're interested in ancient ruins, stunning beaches, or vibrant nightlife, the Rhodes Sightseeing Travel Guide has everything you need to plan an unforgettable trip to this enchanting destination.

  • Vacation Beach and Sightseeing

    Vacation Beach and Sightseeing is a popular travel package that combines relaxation on the beach with exploring nearby attractions. This type of vacation allows travelers to enjoy the sun, sand, and sea while also immersing themselves in the local culture and history of the destination. Whether it's lounging on the beach or visiting landmarks and museums, Vacation Beach and Sightseeing offers a well-rounded travel experience for those looking to unwind and explore.

  • Which travel destination is suitable for a short vacation?

    A travel destination suitable for a short vacation would be a place that is easily accessible and offers a variety of activities in a compact area. For example, a city like New York or Paris would be great for a short vacation, as they have a lot to offer in terms of sightseeing, dining, and entertainment all within a relatively small area. Alternatively, a beach destination like the Caribbean or a mountain resort like Aspen could also be ideal for a short getaway, offering relaxation and outdoor activities in a condensed timeframe. Ultimately, the best destination for a short vacation will depend on the traveler's interests and preferences.

  • Which vacation always gives you wanderlust?

    The vacation that always gives me wanderlust is a trip to Southeast Asia. The vibrant cultures, delicious cuisine, stunning landscapes, and rich history of countries like Thailand, Vietnam, and Indonesia always leave me wanting to explore more. The mix of bustling cities, tranquil beaches, and lush jungles in this region never fails to ignite my sense of adventure and curiosity, making me eager to plan my next journey there.

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